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fluorogenic caspase-3 substrate ac-devd-amc  (Millipore)


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    Structured Review

    Millipore fluorogenic caspase-3 substrate ac-devd-amc
    Fluorogenic Caspase 3 Substrate Ac Devd Amc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorogenic+caspase-3+substrate+ac-devd-amc/caspase+3+substrate+ac+devd+amc/pm32531281-865-21-34
    Average 90 stars, based on 1 article reviews
    fluorogenic caspase-3 substrate ac-devd-amc - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: The Mechanosensitive Ion Channel MSL10 Potentiates Responses to Cell Swelling in Arabidopsis Seedlings.
    Article Snippet: Seedlings were harvested at the indicated time points after cell swelling and caspase-3-like activity measured by determining the cleavage of the fluorogenic caspase-3 substrate Ac-DEVD-AMC using a commercial kit (Caspase 3 Fluorometric Assay Kit, Millipore-Sigma).

    Article Title: Hop proanthocyanidins induce apoptosis, protein carbonylation, and cytoskeleton disorganization in human colorectal adenocarcinoma cells via reactive oxygen species
    Article Snippet: The fluorogenic caspase-3 substrate, Ac-DEVD-AMC [N-acetyl-Asp-Glu-Val-Asp-AMC (7-amino-4-methylcoumarin)], was purchased from EMD Biosciences, Inc. (San Diego, CA, USA).

    Article Title: The Interplay of AMP-activated Protein Kinase and Androgen Receptor in Prostate Cancer Cells
    Article Snippet: Caspase-3 activity assay Fresh-made whole cell extract (20 μg per sample) was incubated with 20 μM fluorogenic caspase-3 substrate Ac-DEVD-AMC (Calbiochem, La Jolla, CA) in 100 μL of Tris-HCl (20 mM, pH 7.5).

    Article Title: The Interplay of AMP-activated Protein Kinase and Androgen Receptor in Prostate Cancer Cells
    Article Snippet: Fresh-made whole cell extract (20 μg per sample) was incubated with 20 μM fluorogenic caspase-3 substrate Ac-DEVD-AMC (Calbiochem, La Jolla, CA) in 100 μL of Tris-HCl (20 mM, pH 7.5).

    Article Title: HPMA as a scaffold for the modular assembly of functional peptide polymers by native chemical ligation.
    Article Snippet: Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands, Eberhard Karls University Tübingen, Department of Molecular Biology, Interfaculty Institute for Cell Biology, Auf der Morgenstelle 15, 72076 Tübingen, Germany, Leibniz Institute for Molecular Pharmacology (FMP), Robert-Rössle-Str.. 10, 13125 Berlin, Germany, Free University Berlin, Institute for Chemistry and Biochemistry, Takustr.. 3, 14195 Berlin, Germany, and EMC microcollections GmbH, Sindelfinger Stra e 3, 72072 Tübingen, Germany.

    Article Title: Protective signalling effect of manganese superoxide dismutase in hypoxia-reoxygenation of hepatocytes.
    Article Snippet: This study investigates the mechanism by which MnSOD exerts its protective effect in hypoxia-reoxygenation (H/R) injury in hepatocytes.. Following induction of H/R, MnSOD expression and activity levels increased and remained high for over 24 h. Hepatocytes silenced for MnSOD (siMnSOD) demonstrated increased susceptibility to H/R-induced apoptotic cell death and a lower capacity to generate mitochondrial reactive oxygen species.. Microarray and real time PCR analysis of gene expression from siMnSOD cells revealed a number of down-regulated protective genes, including hemeoxygenase-1, glutamate-cysteine ligase and Nrf2, a master regulator of cellular adaptation to stress.

    Lysis:

    Article Title: Epidermal growth factor competes with EGF receptor inhibitors to induce cell death in EGFR-overexpressing tumor cells.
    Article Snippet: a Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea b Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea c Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea d Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea e Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea f Department of Physiology and Research Institute for Biomacromolecules, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea

    Article Title: Wip1 suppresses apoptotic cell death through direct dephosphorylation of BAX in response to γ -radiation
    Article Snippet: .. Aliquots of protein (50 μ g) were diluted to 50 μ l with Triton X-100 lysis buffer and then incubated for 1 h with an equal volume of 2 × caspase assay buffer (100 mM HEPES, 10% sucrose, 0.1% CHAPS, 1 mM PMSF, 1 mM DTT, 1 × proteinase inhibitor cocktail) containing 50 μ M of fluorogenic (Ac-DEVD-AMC) caspase-3 substrate (235425; Calbiochem, Billerica, MA, USA). ..

    Incubation:

    Article Title: Epidermal growth factor competes with EGF receptor inhibitors to induce cell death in EGFR-overexpressing tumor cells.
    Article Snippet: a Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea b Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea c Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea d Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea e Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea f Department of Physiology and Research Institute for Biomacromolecules, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea

    Article Title: Wip1 suppresses apoptotic cell death through direct dephosphorylation of BAX in response to γ -radiation
    Article Snippet: .. Aliquots of protein (50 μ g) were diluted to 50 μ l with Triton X-100 lysis buffer and then incubated for 1 h with an equal volume of 2 × caspase assay buffer (100 mM HEPES, 10% sucrose, 0.1% CHAPS, 1 mM PMSF, 1 mM DTT, 1 × proteinase inhibitor cocktail) containing 50 μ M of fluorogenic (Ac-DEVD-AMC) caspase-3 substrate (235425; Calbiochem, Billerica, MA, USA). ..

    Caspase Assay:

    Article Title: Epidermal growth factor competes with EGF receptor inhibitors to induce cell death in EGFR-overexpressing tumor cells.
    Article Snippet: a Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea b Asan Institute for Life Science, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea c Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea d Department of Plastic and Reconstructive Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea e Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea f Department of Physiology and Research Institute for Biomacromolecules, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea

    Article Title: Wip1 suppresses apoptotic cell death through direct dephosphorylation of BAX in response to γ -radiation
    Article Snippet: .. Aliquots of protein (50 μ g) were diluted to 50 μ l with Triton X-100 lysis buffer and then incubated for 1 h with an equal volume of 2 × caspase assay buffer (100 mM HEPES, 10% sucrose, 0.1% CHAPS, 1 mM PMSF, 1 mM DTT, 1 × proteinase inhibitor cocktail) containing 50 μ M of fluorogenic (Ac-DEVD-AMC) caspase-3 substrate (235425; Calbiochem, Billerica, MA, USA). ..



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    The effects of cannabidiol (CBD) on the H 2 O 2 -evoked changes in reactive oxygen species level (ROS, ( A )), mitochondrial membrane potential (MMP, ( B <t>)),</t> <t>caspase-3</t> activity ( C ), and cytotoxicity ( D ) in primary neuronal cell cultures. ( A ) ROS production was assayed with a CM-H 2 DFFDA probe, as described in details found in the Material and Methods section. ( B ) MMP measurement was performed after 6 h of treatment of cells with CBD (0.01–5 μM), NAC (1 mM), and H 2 O 2 (0.2 mM) employing a TMRE fluorescence probe. ( C , D ) Caspase-3 activity and cytotoxicity measurements were performed in cells treated for 9 h with CBD (0.01–5 μM) and H 2 O 2 (0.2 mM). A caspase-3 inhibitor, Ac-DEVD-CHO (Ac, 20 μM), was used as a positive control to the assay. After treatment, in cell lysates, caspase-3 activity ( C ) was measured using the <t>fluorogenic</t> substrate Ac-DEVD-AMC, and cytotoxicity was assessed in the cell culture medium (LDH test, ( D )). Data after normalization to vehicle-treated cells (100%) are presented as a mean ± SEM. * p < 0.05 and *** p < 0.001 vs. vehicle-treated cells; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. H 2 O 2 - treated cells.
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    (A) Representative images of HaCaT human skin keratinocytes incubated with indicated concentrations of salpn for 72 h in 96-well plates. (B) Dose-response for HaCaT skin keratinocytes cells after treatment with salpn showing a marked decrease in cell viability after exposure to salpn. The IC 50 (C) for salpn is 84 μM. Annexin/PI Apoptotic Assay of HaCaT cells treated by salpn for 72 h (C & D). (C) Representative images of HaCaT human skin keratinocytes in 6-well plates showing effects of exposure to increasing concentrations of salpn for 72 h. (D) Annexin-PI staining apoptosis data after treatment with salpn showing % of live cells, early, late and total apoptosis, respectively. <t>Caspase-3</t> Activity Assay of HaCaT Cells treated with increasing concentrations of salpn for 72 h (E) . Induction of caspase-3 activity in Jurkat positive control cells exposed to etoposide (upper panel) and cells treated with different concentrations of salpn (lower panel). Zebrafish morphological assay (F) . Dose-dependent response toxicity of salpn measured as the lowest dose leading to significant developmental abnormalities at 2 days after post fertilization (2 dpf). TUNEL TMR apoptosis assay (G) with salpn treated embryos, dark dim background staining has either no staining (Negative control) or very few apoptotic cells (DMSO control). Arrows on the picture show the positive cells. Apoptotic cells in 2 dpf embryos were labeled using the in-situ Cell Death Detection Kit-TMR.
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    (A) Representative images of HaCaT human skin keratinocytes incubated with indicated concentrations of salpn for 72 h in 96-well plates. (B) Dose-response for HaCaT skin keratinocytes cells after treatment with salpn showing a marked decrease in cell viability after exposure to salpn. The IC 50 (C) for salpn is 84 μM. Annexin/PI Apoptotic Assay of HaCaT cells treated by salpn for 72 h (C & D). (C) Representative images of HaCaT human skin keratinocytes in 6-well plates showing effects of exposure to increasing concentrations of salpn for 72 h. (D) Annexin-PI staining apoptosis data after treatment with salpn showing % of live cells, early, late and total apoptosis, respectively. <t>Caspase-3</t> Activity Assay of HaCaT Cells treated with increasing concentrations of salpn for 72 h (E) . Induction of caspase-3 activity in Jurkat positive control cells exposed to etoposide (upper panel) and cells treated with different concentrations of salpn (lower panel). Zebrafish morphological assay (F) . Dose-dependent response toxicity of salpn measured as the lowest dose leading to significant developmental abnormalities at 2 days after post fertilization (2 dpf). TUNEL TMR apoptosis assay (G) with salpn treated embryos, dark dim background staining has either no staining (Negative control) or very few apoptotic cells (DMSO control). Arrows on the picture show the positive cells. Apoptotic cells in 2 dpf embryos were labeled using the in-situ Cell Death Detection Kit-TMR.
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    BDL in HC fed mice increases apoptosis. Tissue was obtained from Sham and three days after BDL animals, under (a-b) standard Chow control diet (Chow) or (c-d) high cholesterol (HC) diet, and apoptosis was assayed by (e) TUNEL immunohistochemistry; (e) quantification of positive TUNEL cells or by (f) <t>caspase</t> <t>3</t> activity. Images are representative from at least four independent mice. Original magnification: 100x. ∗ p < 0.01 versus Chow fed mice, # p < 0.01 versus 3 days Chow fed mice, and & p < 0.01 versus HC fed mice.
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    Image Search Results


    The effects of cannabidiol (CBD) on the H 2 O 2 -evoked changes in reactive oxygen species level (ROS, ( A )), mitochondrial membrane potential (MMP, ( B )), caspase-3 activity ( C ), and cytotoxicity ( D ) in primary neuronal cell cultures. ( A ) ROS production was assayed with a CM-H 2 DFFDA probe, as described in details found in the Material and Methods section. ( B ) MMP measurement was performed after 6 h of treatment of cells with CBD (0.01–5 μM), NAC (1 mM), and H 2 O 2 (0.2 mM) employing a TMRE fluorescence probe. ( C , D ) Caspase-3 activity and cytotoxicity measurements were performed in cells treated for 9 h with CBD (0.01–5 μM) and H 2 O 2 (0.2 mM). A caspase-3 inhibitor, Ac-DEVD-CHO (Ac, 20 μM), was used as a positive control to the assay. After treatment, in cell lysates, caspase-3 activity ( C ) was measured using the fluorogenic substrate Ac-DEVD-AMC, and cytotoxicity was assessed in the cell culture medium (LDH test, ( D )). Data after normalization to vehicle-treated cells (100%) are presented as a mean ± SEM. * p < 0.05 and *** p < 0.001 vs. vehicle-treated cells; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. H 2 O 2 - treated cells.

    Journal: Biomolecules

    Article Title: Protective Effects of Cannabidiol (CBD) against Qxidative Stress, but Not Excitotoxic-Related Neuronal Cell Damage—An In Vitro Study

    doi: 10.3390/biom14050564

    Figure Lengend Snippet: The effects of cannabidiol (CBD) on the H 2 O 2 -evoked changes in reactive oxygen species level (ROS, ( A )), mitochondrial membrane potential (MMP, ( B )), caspase-3 activity ( C ), and cytotoxicity ( D ) in primary neuronal cell cultures. ( A ) ROS production was assayed with a CM-H 2 DFFDA probe, as described in details found in the Material and Methods section. ( B ) MMP measurement was performed after 6 h of treatment of cells with CBD (0.01–5 μM), NAC (1 mM), and H 2 O 2 (0.2 mM) employing a TMRE fluorescence probe. ( C , D ) Caspase-3 activity and cytotoxicity measurements were performed in cells treated for 9 h with CBD (0.01–5 μM) and H 2 O 2 (0.2 mM). A caspase-3 inhibitor, Ac-DEVD-CHO (Ac, 20 μM), was used as a positive control to the assay. After treatment, in cell lysates, caspase-3 activity ( C ) was measured using the fluorogenic substrate Ac-DEVD-AMC, and cytotoxicity was assessed in the cell culture medium (LDH test, ( D )). Data after normalization to vehicle-treated cells (100%) are presented as a mean ± SEM. * p < 0.05 and *** p < 0.001 vs. vehicle-treated cells; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. H 2 O 2 - treated cells.

    Article Snippet: Caspase-3 (Ac-DEVD-AMC) fluorogenic substrate was from Enzo Life Sciences (New York, NY, USA).

    Techniques: Membrane, Activity Assay, Fluorescence, Positive Control, Cell Culture

    (A) Representative images of HaCaT human skin keratinocytes incubated with indicated concentrations of salpn for 72 h in 96-well plates. (B) Dose-response for HaCaT skin keratinocytes cells after treatment with salpn showing a marked decrease in cell viability after exposure to salpn. The IC 50 (C) for salpn is 84 μM. Annexin/PI Apoptotic Assay of HaCaT cells treated by salpn for 72 h (C & D). (C) Representative images of HaCaT human skin keratinocytes in 6-well plates showing effects of exposure to increasing concentrations of salpn for 72 h. (D) Annexin-PI staining apoptosis data after treatment with salpn showing % of live cells, early, late and total apoptosis, respectively. Caspase-3 Activity Assay of HaCaT Cells treated with increasing concentrations of salpn for 72 h (E) . Induction of caspase-3 activity in Jurkat positive control cells exposed to etoposide (upper panel) and cells treated with different concentrations of salpn (lower panel). Zebrafish morphological assay (F) . Dose-dependent response toxicity of salpn measured as the lowest dose leading to significant developmental abnormalities at 2 days after post fertilization (2 dpf). TUNEL TMR apoptosis assay (G) with salpn treated embryos, dark dim background staining has either no staining (Negative control) or very few apoptotic cells (DMSO control). Arrows on the picture show the positive cells. Apoptotic cells in 2 dpf embryos were labeled using the in-situ Cell Death Detection Kit-TMR.

    Journal: Ecotoxicology and environmental safety

    Article Title: A novel chemo-phenotypic method identifies mixtures of salpn, vitamin D3, and pesticides involved in the development of colorectal and pancreatic cancer

    doi: 10.1016/j.ecoenv.2022.113330

    Figure Lengend Snippet: (A) Representative images of HaCaT human skin keratinocytes incubated with indicated concentrations of salpn for 72 h in 96-well plates. (B) Dose-response for HaCaT skin keratinocytes cells after treatment with salpn showing a marked decrease in cell viability after exposure to salpn. The IC 50 (C) for salpn is 84 μM. Annexin/PI Apoptotic Assay of HaCaT cells treated by salpn for 72 h (C & D). (C) Representative images of HaCaT human skin keratinocytes in 6-well plates showing effects of exposure to increasing concentrations of salpn for 72 h. (D) Annexin-PI staining apoptosis data after treatment with salpn showing % of live cells, early, late and total apoptosis, respectively. Caspase-3 Activity Assay of HaCaT Cells treated with increasing concentrations of salpn for 72 h (E) . Induction of caspase-3 activity in Jurkat positive control cells exposed to etoposide (upper panel) and cells treated with different concentrations of salpn (lower panel). Zebrafish morphological assay (F) . Dose-dependent response toxicity of salpn measured as the lowest dose leading to significant developmental abnormalities at 2 days after post fertilization (2 dpf). TUNEL TMR apoptosis assay (G) with salpn treated embryos, dark dim background staining has either no staining (Negative control) or very few apoptotic cells (DMSO control). Arrows on the picture show the positive cells. Apoptotic cells in 2 dpf embryos were labeled using the in-situ Cell Death Detection Kit-TMR.

    Article Snippet: Caspase-3 assays were performed to measure free 7-amino-4-methylcoumarin (AMC) using 100 μl reactions containing caspase-3 fluorogenic substrate Ac-DEVD-AMC (Invitrogen).

    Techniques: Incubation, Staining, Caspase-3 Activity Assay, Activity Assay, Positive Control, TUNEL Assay, Apoptosis Assay, Negative Control, Labeling, In Situ

    BDL in HC fed mice increases apoptosis. Tissue was obtained from Sham and three days after BDL animals, under (a-b) standard Chow control diet (Chow) or (c-d) high cholesterol (HC) diet, and apoptosis was assayed by (e) TUNEL immunohistochemistry; (e) quantification of positive TUNEL cells or by (f) caspase 3 activity. Images are representative from at least four independent mice. Original magnification: 100x. ∗ p < 0.01 versus Chow fed mice, # p < 0.01 versus 3 days Chow fed mice, and & p < 0.01 versus HC fed mice.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Liver Cholesterol Overload Aggravates Obstructive Cholestasis by Inducing Oxidative Stress and Premature Death in Mice

    doi: 10.1155/2016/9895176

    Figure Lengend Snippet: BDL in HC fed mice increases apoptosis. Tissue was obtained from Sham and three days after BDL animals, under (a-b) standard Chow control diet (Chow) or (c-d) high cholesterol (HC) diet, and apoptosis was assayed by (e) TUNEL immunohistochemistry; (e) quantification of positive TUNEL cells or by (f) caspase 3 activity. Images are representative from at least four independent mice. Original magnification: 100x. ∗ p < 0.01 versus Chow fed mice, # p < 0.01 versus 3 days Chow fed mice, and & p < 0.01 versus HC fed mice.

    Article Snippet: For each reaction, caspase 3 synthetic fluorogenic tetrapeptide substrate (Ac-DEVD-AMC) was added (BD Pharmingen) and then the tissue lysate; after that samples were incubated for one hour.

    Techniques: TUNEL Assay, Immunohistochemistry, Activity Assay